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KMID : 1011820190600020091
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Investigative and Clinical Urology
2019 Volume.60 No. 2 p.91 ~ p.98
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Characterization of human infiltrating and circulating gamma-delta T cells in prostate cancer
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Vella Marco
Coniglio Daniela
Abrate Alberto
Gesolfo Cristina Scalici
Lo Presti Elena
Meraviglia Serena
Serretta Vincenzo
Simonato Alchiede
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Abstract
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Purpose: The aim of our study was to prospectively evaluate the distribution of gamma-delta (γδ)1 and γδ2 T cells and their phenotypes in peripheral blood and prostate samples of patients diagnosed with or without prostate cancer (PCa) at prostate biopsy.
Materials and Methods: A consecutive series of 43 outpatients underwent trans-rectal echo-guided prostate biopsy for suspected PCa. Flow cytometry analysis was used to identify and characterize the γδ T cells populations in peripheral blood and tissue samples. Patients were stratified according to the presence or not of PCa, and its International Society of Urological Pathology (ISUP) grade (1 vs. ≥2).
Results: The distribution of γδ T cells in peripheral blood and prostate tissue showed wide variability and non-significant differences. A slightly higher percentage of δ2 T cells and a slightly lower percentage of δ1 T cells were found in peripheral blood of cancer patients. A non-significantly higher percentage of both Vδ1 and Vδ2 was expressed in cancer tissues, but a trend for lower distribution of δ1 and δ2 T cells was observed in ISUP grade ≥2. The “central memory” and “effector memory” were the most expressed T cells phenotype in peripheral blood and tissue samples. However no substantial differences in T cells subtypes distribution between cancer and healthy tissue were observed.
Conclusions: No substantially different percentages of γδ T cells were found in peripheral blood and biopsy samples of healthy and PCa patients. However a non-significant trend for lower infiltrate in higher ISUP grade cancer tissue was observed, suggesting a possible role for the immunosurveillance of PCa.
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KEYWORD
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Biopsy, Immunologic surveillance, Neoplasm grading, Prostatic neoplasms, T-Lymphocyte subsets
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